We’ve released our ChIP-Seq workflow this week, available to anyone with a Free Basic Account inside of GenomeQuest. Like all of our NGS workflows, it runs in two basic steps: a mapping step and a downstream analysis step. In this case, of course, the downstream analysis is a peak-finding algorithm. We chose the MACS modeling software for peak modeling. (You can see the entire workflow’s documentation here.) Integrated into the GenomeQuest Sequence Data Management platform, it outputs a heavily annotated sequence database, which can then be interactively filtered, grouped, sorted, and mined for peaks of interest. And this can all be connected to your RNA-Seq and resequencing data to get the global picture.
So now researchers can go from their ChIP-Seq NGS runs directly to gene-based annotation of the peaks found by their biology. Select regions of interest, or genes of interest, or peaks of a certain class, and drill down to see the actual evidence that backs up the call.
We’re giving away free ChIP-Seq runs to the first 100 people to sign up.
As always, feel free to leave a comment – we read every one.
Richard J. Resnick
VP Software and Services